Our inflammation model recreates acute or chronic inflammatory states in human endothelial cells. This platform enables evaluation of compound efficacy and a reliable way to study inflammatory responses in a vascular micro-environment.

Multiple desease models available

Acute persistent inflammation

› Non-alcoholic Steatohepatitis (NASH)
› Western Diet Obesity
› Atherosclerosis
› Diabetes-II

Chronic non-resolving inflammation

› Multiple Sclerosis
› Rheumatoid Arthritis
› Inflammatory Bowel Disease

Vascular pathologies

› Sickle Cell Disease
› Vascular Occlusion
› Ischemia

Applications & Readouts

Anti-inflammatory drug development

› Therapeutic and prophylactic protocols available
› Multi-throughput screening
› Dose response analysis and IC50 determination

Mechanism of Action (MoA) studies

› Analysis of endothelial activation pathways
› Measurement of adhesion molecules and cytokines
› Identification of pro- or anti-inflammatory effects

Vascular modeling

› Regular vascular flow: leukocytes trafficking measurements (capture, rolling, migration and transmigration)
› Disruptive flow: monocytes or neutrophils platelet aggregates
‍› Rolling leukocyte flow: endothelial cells replaced by platelets‍

Chronic and acute disease modeling

› Acute-persistent inflammation:
• Neutrophil extra cellular traps (NETs)
• Oxidized low-density lipoprotein (OxLDL)
• Tumor necrosis factor superfamily (TNFSF14)
‍

› Chronicnon-resolving inflammation:
• Tumor necrosis factor alpha (TNFα)
• Interfer on gamma (IFNɣ)
• Lipopolysaccharides (LPS)

Safety and toxicity assessment

› Endothelial cell activation and dysfunction

› Endothelial junctions integrity

Assays & Methods

Bio-imaging

› High-resolution imaging
by phase contrastmicroscopy

› Immunohistochemistry: visualisation of cellular morphology, drug-specific effect
‍
› Adjustable time-course measurements

Cell tracking

› MesenCount and MesenTrack AI-based softwares

› Label-free or immunofluorescence imaging
under flow

Flow Cytometry

› Recovery and phenotyping of recruited leukocyte subsets in whole blood models
‍
› Identification of endothelial cell activation markers

› Measurement of cytokines production

Example data

Example 1

Trafficking of human monocytes on activated HUVECs under flow

Trafficking of human monocytes on activated HUVECs under flow. Monocytes flowed over HUVECs for 5-mins (area marked in grey). They were tracked, andtheir positions marked at 1-min intervals. Monocytes captured from free-flow became rapidly activated by rolling on the luminal surface, changing phase from white(squares) to grey (triangles). Monocytes that underwent transmigration into the ablumen changed to phase-black (inverted triangles).

Example 2

Blockade in Monocyte transmigration identified with Compound-C
Success with a typical screening strategy

Monocyte transmigration on the surface of activated HUVECs under flow. Primary human monocytes co-cultured on TNFα-activated HUVECs. Test compounds at 30 µM or control were added to cells for 45 min and the transmigration blockade recorded.

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